Name the three main steps involved in recombinant DNA technology?
What is a restriction enzyme?
What are the bacteria used in your protocol?
Explain the calcium/phosphate (heat-shock) method and what it is used for.
How can we selectively grow bacteria that have taken up the plasmid?
Explain the principle behind blue and white screening and its purpose in this lab.
What are the sites the plasmid contains that allow for this experiment?
What are the 3 compounds in the LB plates that allow for selection of bacteria with plasmid and the distinction of plasmids with and without the inserted gene?
What are the six steps in plasmid preparation and purification?
What is a restriction enzyme and why was it used in this experiment?
Explain the principle of Gel Electrophoresis, what is it for?
What´s the compound that allows for the visualization of the DNA in the gel?
How many times does BamHI cut the plasmid without the insert?
And the plasmid with the insert?
Explain the different plasmid conformations that exist. Förståelse Biokemi / Plasmidlabb /