Plasmid DNA purified from colonies grown on blue/white screening plates was verified by electrophoresis. Samples in Lanes C and D are digested by enzyme for verification whilst Lanes A and B are non-digested samples. Select the correct description/descriptions based on the gel picture below.
Välj ett eller flera alternativ:
- A: The band in Lane A that migrates the furthest is in supercoiled form and we can predict its size by a comparison with the size markers.
- B: The sample in Lane C has the largest size of DNA because it displays the brightest intensity under UV light.
- C: By comparing Lanes C and D, we can infer that the sample in Lane D is a plasmid with an insert DNA whilst Lane C is an empty vector.
- D: By comparing Lanes A and B, we can infer that the sample in Lane B is a plasmid with an insert DNA whilst Lane A is an empty vector.
Rätt svar
- C
- D
(Flersvarsfråga; i originalbilden är första påståendet felmarkerat, de två sista rättmarkerade.)